| Job Description: |
This is an intermediate level Laboratory Technician position in a research lab developing immune based therapies for pediatric cancer. The Laboratory Technician is expected to be able to 1. Complete the execution of assigned experiments; 2. Assist in maintaining a necessary rodent colony and performing rodent procedures (i.e. tumor implantation, tail vein injection, intraperitoneal injection, etc, either unsupervised or following training) to complete research objectives 3. Present researching findings to PI and at laboratory meetings; and 4. Maintain laboratory safety programs and train personnel in laboratory safety techniques
The Laboratory Technician II I must be able to perform the following essential functions:
- Animal Studies: Handling mice for setting up timed matings, weaning animals and colony maintenance. Participating in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., i.v., or intracranial injection. Other duties with the mice may include doing echocardiography, sensory and motor function, force mechanics, and metabolic studies. Plays a key role in regulatory management (IACUCs).
- Histological Work/Analysis: Collecting organs and other tissues and embedding the specimens for sectioning either with a microtome or cryotome (for frozen sections). Following the preparation of slides with sections tissues, staining with standard histology stains such as H&E, as well as with immunohistochemistry will be done. Conduct image capture and analysis of tissue following intervention studies.
- Molecular Techniques and Analysis: Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines; immunoassays to gene therapy vectors and transgenes, including ELISAs. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
- Regulatory Compliance: Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met. Train new laboratory members to ensure compliance standards are met.
- Training: Train and supervise graduate and undergraduate students performing diagnostic procedures related to gene therapy experiments
- Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include performing tasks and experiments as directed by the Principal Investigator
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| Preferred Qualifications: |
- Animal colony management and handling of rodents experience (highly preferred)
- Experience maintaining cell lines, performing plasmid transfections/viral infections, immunohistochemistry, FACS, rt-PCR, genotyping, immunohistochemistry, western blotting, microscopy
- Experience administering treatments i.v., i.p., i.m., and/or intracranially to rodents
- Working knowledge of basic laboratory techniques
- Must be extremely knowledgeable in laboratory procedures and must be meticulous in recording, tracking & reporting of data collected
- Strong interest in translational cancer research
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| Special Instructions to Applicants: |
In order to be considered, you must upload your cover letter and resume.
This position is eligible for veteran’s preference. If you are claiming veteran’s preference, please upload a copy of your DD 214 Member Copy 4 with your application for consideration. See our Veteran's Preference Page for more specific information.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.
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