Biological Scientist II - Hematology Oncology

  • University of Florida
  • United States
  • May 25, 2023
Full Time - Continuing

Job Description:

Classification Title:

Biological Scientist II

Job Description:

The Biological Scientist II position described herein performs research in the laboratory of Dr. Paul Castillo Department of Pediatrics, Hematology Oncology. With minimal direction, performs a variety of molecular based laboratory research and clerical tasks determined by the field and scope of the particular research study. May perform tasks related to the research project independently, within broad guidelines and subject to periodic review by supervisor or other research staff.

This is an intermediate level Biological Scientist position in a research lab developing immunotherapies against cancers. The Biological Scientist is expected to be able to 1. modify protocols to improve existing techniques or to incorporate new diagnostic procedures; 2. Complete the execution of assigned experiments; 3. Assist in maintaining a necessary rodent colony to achieve research objectives; 4. Assist in performing rodent procedures as necessary to complete research objectives 5. Present researching findings to PI and at laboratory meetings; and 8. Maintain laboratory safety programs and train personnel in laboratory safety techniques
The Biological Scientist II must be able to perform the following essential functions:
Histological Work/Analysis:
Collecting organs and other tissues and embedding the specimens for sectioning either with a microtome or cryotome (for frozen sections). Following the preparation of slides with sections tissues, staining with standard histology stains such as H&E, as well as with immunohistochemistry will be done. Conduct image capture and analysis of tissue following intervention studies.
Molecular Techniques and Analysis:
Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, Isolating RNA and performing real-time PCR, and PCR genotyping of transgenic mouse lines; immunoassays to immunotherapy therapy gene therapy vectors and transgenes, including ELISAs. This may include expansion/maintenance of cell lines and analysis of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue lysates and performing/analyzing western blots.
Animal Studies:
Handling mice for setting up timed matings, weaning animals, and colony maintenance. Participating in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v. injection. Plays a key role in regulatory management (IACUCs).
Regulatory Compliance:
Ensures the laboratory is compliant with governing rules and regulations. Coordinate all IACUC and IRB (exempt) submissions/renewals. Ensure all EH&S standards are met. Train new laboratory members to ensure compliance standards are met.
Train and supervise graduate and undergraduate students performing diagnostic procedures related to immune therapy experiments.
Any other duties, as needed, to fulfill the mission and to abide by the values of the College of Medicine. This will include performing tasks and experiments as directed by the Principal Investigator
Expected Salary:

$45,000 - $50,000 annually; commensurate within this range based on qualifications and experience.

Minimum Requirements:

Bachelor’s degree in biology or a closely related field and three years of relevant experience; or a Master’s degree in biology or a closely related field and one year of relevant experience; Doctoral degree in biology or a closely related field.

Preferred Qualifications: Demonstrated abilities in laboratory techniques required. Exposure to applicable computer techniques, including specific software application, is required. Appropriate communication skills required.
Special Instructions to Applicants:

To be considered for this position, please upload your cover letter or letter of interest, resume, and a list of professional references.

Monday through Friday, 8:00am to 5:00pm. Nights & weekends as needed for on-going research.

The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.

This is a time-limited position.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required: Yes