Biological Scientist I

 
  • University of Florida
  • United States
  • Sep 21, 2022
Full Time - Continuing

Job Description:

Classification Title:

Biological Scientist I

Job Description:

Flow cytometry Staining and Analyzing

  • Lead and assist other scientists with the development, optimization, and validation of flow cytometry assays and panels.
  • Perform data analysis and troubleshoot technical issues relating to assay development and quality.
  • Prepare primary cells for surface & intracellular antibody staining.
  • Acquire data and perform analysis using BD Fortessa, BD AriaIII, and Cytek Aurora, BioRad S3e multicolor flow cytometers.
  • Perform QC and validation of the multicolor flow cytometers.
  • Process and sort primary cell populations.
  • Perform complete blood count testing and cell viability counting.
  • Maintain accurate and complete records.
  • Analyze the flow cytometry data with FlowJo software.
  • Prepare analyzed data to present for various projects.
  • Develop and standardize new protocols for new projects
  • Develop new data management protocols in UF approved storage systems to store raw data and analyzed data

Tissue Processing & cell isolation

  • Perform all aspects of processing, expanding, and cryopreservation of tissue such as blood, spleen, bone marrow, and thymus to establish a better understanding of immune system heterogeneity and cellular diversity within tissues.
  • Process and Isolate PBMC and other specific cell populations from leukopaks and blood collections.
  • Perform cryopreservation of cells, aliquoting and standard tissue culture procedures
  • Ensure samples are returned to appropriate storage and updating sample status in database
  • Standardize and streamline validated protocols to increase efficiency

DNA & RNA Isolation

  • Perform gel Extraction to isolate pure DNA for sequencing
    • Perform RNA/DNA cleaning, concentration, and quantification protocols.
    • Prepare cDNA libraries for RT-PCR or sequencing.

    ELISA

    • Perform ELISA assays to detect and quantify substances such as peptides, proteins, and antibodies.
    • Optimize ELISA by systematically adjusting and testing the many components and variables to help ensure results are robust and accurate. Cell Culture 10%
    • Process, expand, and cryopreserve primary tissues such as blood, spleen, bone marrow, and thymus to establish a better understanding of immune system heterogeneity and cellular diversity within tissues
    • Prepare media for Jurkat E6-1 cells, BLox5 cells, NK-92 cells, and K-562 cells  Perform the cell line expansion of primary human cells and immortal cells.

    qPCR Amplification

    • Generate multiple copies of a target from a single molecule of DNA or mRNA via an in vitro amplification method to detect individual genes or specific DNA regions or mutations of interest.
    • Perform Dye-based quantitative PCR (qPCR) and Probe-based quantitative PCR (qPCR).

    Training new technicians

    • Train new staff in the operations of flow cytometers and software packages associates with data analysis.
    • Act as a source of technical expertise for others.
    • Act as a team leader and assist in training others on SOPs and quality guidelines.
    • Select training/instructional methods and procedures appropriate for the situation when learning or teaching new things
Expected Salary:

$19.15 - $21.55 per hour

Minimum Requirements:

Bachelor' s degree in biology or a closely related life science field.

Preferred Qualifications:

Experience with genetics, immunology, and/or cell biology research techniques in a research or clinical laboratory

Demonstrated ability to think critically and cover a broad range of responsibilities.

Must be self-motivated and highly responsible, with the ability to execute technical tasks and projects with minimal supervision and work multiple projects simultaneously.

Strong written and verbal communications skills.

18 months of full-time laboratory experience.

Experience in biological sample handling, processing, staining, and analysis via flow.

Flow cytometry experience with thorough understanding of life science applications of flow cytometry.

Knowledge of cell sorting / panel design & optimization.

Ability to determine root causes and develop solutions.

Desire to expand knowledge flow cytometric methods and learn to design flow cytometry experiments to support immunology programs.

Special Instructions to Applicants:

In order to be considered, you must upload your cover letter and resume.

The University of Florida is committed to non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, gender identity and expression, marital status, national origin, political opinions or affiliations, genetic information and veteran status in all aspects of employment including recruitment, hiring, promotions, transfers, discipline, terminations, wage and salary administration, benefits, and training.

This is a time limited position.

This position is eligible for veteran’s preference. If you are claiming veteran’s preference, please upload a copy of your DD 214 Member Copy 4 with your application for consideration. See our Veteran's Preference Page for more specific information.

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required:Yes